{"id":15,"date":"2023-01-24T10:44:42","date_gmt":"2023-01-24T01:44:42","guid":{"rendered":"https:\/\/www.eiiris.tut.ac.jp\/numano\/?page_id=15"},"modified":"2023-01-25T19:38:29","modified_gmt":"2023-01-25T10:38:29","slug":"research","status":"publish","type":"page","link":"https:\/\/www.eiiris.tut.ac.jp\/numano\/research\/","title":{"rendered":"\u7814\u7a76\u5185\u5bb9"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">\u7814\u7a76\u5185\u5bb9<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">\u54fa\u4e73\u985e\u6982\u65e5\u30ea\u30ba\u30e0\u306e\u5206\u5b50\u6642\u8a08\u306e\u30e2\u30cb\u30bf\u30fc\u5b9f\u9a13\u7cfb\u306e\u78ba\u7acb<\/h3>\n\n\n\n<h4 class=\"wp-block-heading\"><strong>Per1\uff1aluc\u306e\u7d44\u307f\u63db\u3048\u52d5\u7269\u306eSCN\u306e\u767a\u5149\u30ea\u30ba\u30e0<\/strong><\/h4>\n\n\n\n<p>\u5730\u7403\u4e0a\u306e\u751f\u7269\u306e\u884c\u52d5\u3084\u4ee3\u8b1d\u306a\u3069\u306b\u306f\u7d04\u4e00\u65e5\u3092\u5468\u671f\u3068\u3059\u308b\u4f53\u5185\u6642\u8a08\u304c\u5236\u5fa1\u3059\u308b\u6982\u65e5\u30ea\u30ba\u30e0\u304c\u5b58\u5728\u3057\u3001\u305d\u306e\u4f4d\u76f8\u306f\u5916\u754c\u306e\u5149\u74b0\u5883\u306a\u3069\u306b\u3088\u308a\u5909\u5316\u3059\u308b\u3002\u54fa\u4e73\u985e\u306e\u6982\u65e5\u30ea\u30ba\u30e0\u306e\u4e2d\u67a2\u306f\u8996\u4ea4\u53c9\u4e0a\u6838(SCN\uff09\u306b\u5b58\u5728\u3059\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u3066\u3044\u308b\u304c\u3001\u30ea\u30ba\u30e0\u306e\u30aa\u30b7\u30ec\u30fc\u30bf\u30fc\u6a5f\u80fd\u306b\u304a\u3044\u3066Period1(Per1)\u907a\u4f1d\u5b50\u306eSCN\u306b\u304a\u3051\u308b\u767a\u73fe\u632f\u52d5\u304c\u91cd\u8981\u3067\u3042\u308b\u3053\u3068\u3001\u307e\u305f\u3001\u30ea\u30ba\u30e0\u306e\u5149\u74b0\u5883\u3078\u306e\u540c\u8abf\u306fPer1\u306e\u8ee2\u5199\u306e\u5149\u8a98\u5c0e\u306b\u7531\u6765\u3059\u308b\u3053\u3068\u3092\u3053\u308c\u307e\u3067\u306b\u793a\u3057\u305f\u3002\u3053\u306ePer1\u306e\u767a\u73fe\u3092\u6307\u6a19\u306b\u3057\u3066\u3001\u4e2d\u67a2SCN\u53ca\u3073\u672b\u68a2\u7d44\u7e54\u306e\u6642\u8a08\u6a5f\u80fd\u3092\u540c\u6642\u306b\u6e2c\u5b9a\u3059\u308b\u305f\u3081\u3001Per1\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u767a\u73fe\u5236\u5fa1\u9818\u57df\u3067\u86cd\u306e\u767a\u5149\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc(luc)\u907a\u4f1d\u5b50\u3092\u767a\u73fe\u3059\u308bPer1::luc \u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cb\u30c3\u30af\u52d5\u7269\u3092\u4f5c\u3063\u305f\u3002\u305d\u306e\u5404\u7d44\u7e54\u3092\u57f9\u990a\u3059\u308b\u3068\u3001SCN\u30b9\u30e9\u30a4\u30b9\u57f9\u990a\u3067\u306f\u3001\u7d041\u65e5\u5468\u671f\u306e\u5316\u5b66\u767a\u5149\u632f\u52d5\u304c\uff11\u30f6\u6708\u4ee5\u4e0a\u89b3\u5bdf\u3055\u308c\u3001\u4e00\u65b9\u3001\u809d\u81d3\u306a\u3069\u672b\u68a2\u7d44\u7e54\u3067\u3082\u9045\u308c\u305f\u632f\u52d5\u4f4d\u76f8\u3067\u306f\u3042\u308b\u304c\u767a\u5149\u632f\u52d5\u304c\u89b3\u5bdf\u3067\u304d\u30012\u65e5\u304b\u30896\u65e5\u3067\u6e1b\u8870\u3059\u308b\u3002\u54fa\u4e73\u985e\u3067\u306fSCN\u306b\u81ea\u5f8b\u3057\u305f\u30da\u30fc\u30b9\u30e1\u30fc\u30ab\u30fc\u304c\u5b58\u5728\u3057\u3001\u672b\u68a2\u306e\u6982\u65e5\u30ea\u30ba\u30e0\u3092\u7d44\u7e54\u56fa\u6709\u306e\u4f4d\u76f8\u306b\u7dad\u6301\u3059\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u3002\u73fe\u5728\u306f\u3001Per1::luc\u30de\u30a6\u30b9\u306a\u3069\u3092\u7528\u3044\u3066\u3001SCN\u30b9\u30e9\u30a4\u30b9\u3092\u9855\u5fae\u93e1\u3068CCD\u30ab\u30e1\u30e9\u3092\u7d44\u307f\u5408\u308f\u305b\u305f\u30bf\u30a4\u30e0\u30e9\u30d7\u30b9\u8a08\u6e2c\u306b\u3066\uff13\u6b21\u5143\u306e\u753b\u50cf\u3068\u3057\u3066\u89b3\u5bdf\u3057\u3066\u3044\u308b\u3002\u307e\u305f\u3001Vapor-liquid-solid(VLS)\u6cd5\u3067\u4f5c\u6210\u3055\u308c\u305f\u5fae\u5c0f\u523a\u5165\u578b\u30de\u30eb\u30c1\u96fb\u6975\u30d7\u30ed\u30fc\u30d6\u3092\u30a2\u30ec\u30a4\u72b6\u306b\u4e26\u3079\u305f\u30bb\u30f3\u30b5\u30fc\u30c1\u30c3\u30d7(\u8c4a\u6a4b\u30d7\u30ed\u30fc\u30d6)\u3092\u7528\u3044\u3066\u3001SCN\u8133\u30b9\u30e9\u30a4\u30b9\u3092\u57f9\u990a\u3057\u306a\u304c\u3089\u3001\u3067\u304d\u308b\u3060\u3051\uff11\u795e\u7d4c\u7d30\u80de\u306b\u8fd1\u3044\u5358\u4f4d\u3067\u523a\u6fc0\u3092\u3057\u306a\u304c\u3089\u3001\uff11\u7d30\u80de\u306e\u795e\u7d4c\u6d3b\u52d5\u30b9\u30d1\u30a4\u30af\u306e\u767a\u706b\u983b\u5ea6\u3092\u6e2c\u5b9a\u3059\u308b\u3002\u3055\u3089\u306b\u3001\u3053\u3046\u3057\u305f\u3001\u5916\u90e8\u304b\u3089\u306e\u523a\u6fc0\u306b\u3088\u308a\u3001SCN\u8133\u30b9\u30e9\u30a4\u30b9\u5185\u306724\u6642\u9593\u30ea\u30ba\u30df\u30c3\u30af\u306b\u89b3\u5bdf\u3055\u308c\u308b\u907a\u4f1d\u5b50\u767a\u73fe\u306e\u5909\u5316\u306b\u3069\u306e\u3088\u3046\u306a\u5f71\u97ff\u3092\u304d\u305f\u3059\u304b\u89b3\u5bdf\u3059\u308b\u3002\u307e\u305f\u3001\u3053\u306e\u8c4a\u6a4b\u30d7\u30ed\u30fc\u30d6\u306f\u3001\u305d\u306e\u7d30\u3055\u304b\u3089\u5b9f\u9a13\u52d5\u7269\u306b\u5bfe\u3057\u3066\u8d85\u4f4e\u4fb5\u8972\u3067\u3042\u308b\u305f\u3081\u3001\u9577\u671f\u306b\u308f\u305f\u308a\u8133\u8a08\u6e2c\u306a\u3069\u304c\u53ef\u80fd\u3067\u3042\u308b\u305f\u3081\u3001\u7d44\u307f\u63db\u3048\u30de\u30a6\u30b9\u3084\u6982\u65e5\u30ea\u30ba\u30e0\u306a\u3069\u306e\u9577\u671f\u8a08\u6e2c\u306b\u6700\u9069\u3067\u3042\u308b\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">\u4e3b\u306a\u696d\u7e3e<\/h4>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Resetting central and peripheral circadian oscillators in transgenic rats.<br>*Yamazaki S, * Numano R, *Abe M, Hida A, Takahashi R, Ueda M, Block GD, Sakaki Y, Menaker M, Tei H.Science, 288, 2000, p682-685 *equally contributed<\/li>\n\n\n\n<li>Constitutive expression of the Period1 gene impairs behavioral and molecular circadian rhythms.<br>Numano R, Yamazaki S, Umeda N, Samura T, Sujino M, Takahashi R, Ueda M, Mori A, Yamada K, Sakaki Y, Inouye ST, Menaker M, Tei H.Proc Natl Acad Sci U S A, 103, 2006, p3716-3721<\/li>\n\n\n\n<li>Nanoscale tipped microwire arrays enhance electrical trap and depth injection of nanoparticles.<br>Goryu A, Numano R, Ikedo A,Ishida M, Kawano T.,<br>Nanotechnology Vol.23, 41, 2012<\/li>\n\n\n\n<li>In vivo bioluminescence and reflectance imaging of multiple organs in bioluminescence reporter mice by bundled-fiber-coupled microscopy<br>Ando Y, i Sakurai T, Koida K, Tei H, Hida A, Nakao K, Natsume M, and Numano R,<br>Biol.I Optics Eexp., 7(3), 963-978, 2016<\/li>\n\n\n\n<li>Single 5\u2009\u03bcm diameter needle electrode block modules for unit recordings in vivo.<br>Sawahata H, Yamagiwa S, Moriya A, Dong T, Oi H, Ando Y, Numano R, Ishida M, Koida K, Kawano<br>T.Scientific Reports, 2016, 25;6:35806<\/li>\n<\/ol>\n\n\n\n<h4 class=\"wp-block-heading\">\u30ad\u30fc\u30ef\u30fc\u30c9<\/h4>\n\n\n\n<p>\u6982\u65e5\u30ea\u30ba\u30e0\u3001Period1\u3001\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cb\u30c3\u30af\u52d5\u7269\u3001\u767a\u73fe\u632f\u52d5\u3001\u8c4a\u6a4b\u30d7\u30ed\u30fc\u30d6\u3001VLS\u6cd5\u3001 \u30da\u30fc\u30b9\u30e1\u30fc\u30ab\u30fc\u795e\u7d4c<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\u7167\u5c04\u3059\u308b\u5149\u306e\u6ce2\u9577\u306b\u3088\u308a\u795e\u7d4c\u6d3b\u52d5\u3092\u5916\u90e8\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3067\u304d\u308b\u5b9f\u9a13\u7cfb\u306e\u78ba\u7acb<\/h3>\n\n\n\n<h4 class=\"wp-block-heading\">\u5149\u7167\u5c04\u306b\u3088\u308b\u795e\u7d4c\u7d30\u80de\u306e\u6d3b\u52d5\u96fb\u4f4d\u5909\u5316<\/h4>\n\n\n\n<p>\u795e\u7d4c\u7d30\u80de\u306e\u30a4\u30aa\u30f3\u30c1\u30e3\u30cd\u30eb\u306e\u6d3b\u52d5\u3092\u3001\u5149\u7167\u5c04\u3067\u30b3\u30f3\u30db\u30fc\u30e1\u30b7\u30e7\u30f3\u3092\u5909\u5316\u3055\u305b\u308b\u5149\u611f\u53d7\u6027\u306e\u4eba\u5de5\u5316\u5b66\u7269\u8cea\u3092\u7528\u3044\u3066\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3059\u308b\u7cfb\u3092\u958b\u767a\u3057\u305f\u3002\u30b0\u30eb\u30bf\u30df\u30f3\u53d7\u5bb9\u4f53(iGluR6)\u306f\u795e\u7d4c\u7d30\u80de\u306e\u30b7\u30ca\u30d7\u30b9\u90e8\u4f4d\u306b\u5f37\u304f\u767a\u73fe\u3057\u3001\u795e\u7d4c\u767a\u706b\u306e\u983b\u5ea6\u3092\u5236\u5fa1\u3057\u3066\u3044\u308b\u30a4\u30aa\u30f3\u30c1\u30e3\u30cd\u30eb\u3067\u3042\u308b\u3002UV\u9818\u57df\u306e\u5149\u3067\u30b7\u30b9\u4f53\u3068\u306a\u308a\u3001\u53ef\u8996\u9818\u57df\u306e\u5149\u3067\u30c8\u30e9\u30f3\u30b9\u4f53\u3068\u306a\u308b\u30a2\u30be\u30d9\u30f3\u30bc\u30f3\u69cb\u9020\u3092\u9aa8\u683c\u306b\u3082\u3061\u3001\u30b7\u30b9\u30c6\u30a4\u30f3(Cys)\u306b\u6c34\u7d20\u7d50\u5408\u3059\u308b\u30de\u30ec\u30fc\u30de\u30a4\u30c9(\u306e\u308a\u3057\u308d)\u69cb\u9020\u4f53\u3068iGluR6\u53d7\u5bb9\u4f53\u306e\u30a2\u30b4\u30cb\u30b9\u30c8\u3067\u3042\u308b\u30b0\u30eb\u30bf\u30df\u30f3\u9178\u3092\u4e21\u65b9\u7d50\u5408\u3055\u305b\u305f\u65b0\u898f\u5316\u5b66\u7269\u8cea(MAG)\u3092\u5408\u6210\u3057\u305f\u3002iGluR6\u306e\u30ea\u30ac\u30f3\u30c9\u7d50\u5408\u90e8\u4f4d\u306e\u307e\u308f\u308a\u306b\u5316\u5408\u7269\u306e\u63a5\u7740\u90e8\u3068\u306a\u308bCys\u5909\u7570\u3092\u5c0e\u5165\u3057\u305f\u5909\u7570\u4f53\u3068\u5316\u5b66\u7269\u8ceaMAG\u306e\u7d44\u307f\u5408\u308f\u305b\u306b\u3088\u308a\u3001\u7570\u306a\u308b\u6ce2\u9577\u306e\u5149\u7167\u5c04\u3067\u3001\u7d30\u80de\u819c\u306e\u96fb\u4f4d\u30fb\u795e\u7d4c\u7d30\u80de\u306e\u6d3b\u52d5\u3092\u53ef\u9006\u7684\u306b\u5909\u5316\u3055\u305b\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u305f\u3002\u5177\u4f53\u7684\u306b\u306f\u3001UV\u9818\u57df\u306e\u5149\u7167\u5c04\u3067\u53d7\u5bb9\u4f53\u304c\u958b\u304d\u3001\u53ef\u8996\u5149\u3067\u9589\u307e\u308b\u3068\u3044\u3046\u5149\u53cd\u5fdc\u6027\u3092\u3057\u3081\u3059\u7d44\u63dbLiGluR\u53d7\u5bb9\u4f53\u3084\u3001\u53cd\u5bfe\u306bUV\u5149\u3067\u53d7\u5bb9\u4f53\u304c\u9589\u307e\u308a\u3001\u53ef\u8996\u5149\u3067\u958b\u304f\u53cd\u5fdc\u3092\u793a\u3059Yin\/Yang\u53d7\u5bb9\u4f53\u3092\u898b\u51fa\u3057\u305f\u3002\u3053\u306e\u5b9f\u9a13\u7cfb\u306f\u30bc\u30d6\u30e9\u30d5\u30a3\u30c3\u30b7\u30e5\u306a\u3069\u306e\u751f\u4f53\u5185\u3067\u3082\u6a5f\u80fd\u3057\u3001\u5916\u90e8\u304b\u3089\u306e\u7167\u5c04\u5149\u306b\u3088\u308a\u795e\u7d4c\u6d3b\u52d5\u30fb\u884c\u52d5\u3092\u8abf\u7bc0\u3067\u304d\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u305f\u3002\u3053\u306eLiGluR\u53d7\u5bb9\u4f53\u3084Yin\/Yang\u53d7\u5bb9\u4f53\u3092\u3082\u3061\u3044\u3066\u3001SCN\u30da\u30fc\u30b9\u30e1\u30fc\u30ab\u30fc\u795e\u7d4c\u306e\u795e\u7d4c\u6d3b\u52d5\u3092\u5149\u7167\u5c04\u3067\u76f4\u63a5\u64cd\u4f5c\u3059\u308b\u3053\u3068\u3067\u3001\u54fa\u4e73\u985e\u6982\u65e5\u30ea\u30ba\u30e0\u3092\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3057\u3066\u3044\u308b\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">\u4e3b\u306a\u696d\u7e3e<\/h4>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Allosteric control of an ionotropic glutamate receptor with an optical switch.<br>Volgraf M, Gorostiza P, Numano R, Kramer RH, Isacoff EY, Trauner D.<br>Nat Chem Biol., 2, 2006, p47-52<\/li>\n\n\n\n<li>Remote control of neuronal activity with a light-gated glutamate receptor.<br>Szobota S, Gorostiza P, Del Bene F, Wyart C, Fortin DL, Kolstad KD, Tulyathan O, Volgraf M, Numano R, Aaron HL, Scott EK, Kramer RH, Flannery J, Baier H, Trauner D, Isacoff EY.<br>Neuron, 54, 2007, p535-545<\/li>\n\n\n\n<li>Mechanisms of photoswitch conjugation and light activation of an ionotropic glutamate receptor.<br>Gorostiza P, Volgraf M, Numano R, Szobota S, Trauner D, Isacoff EY.<br>Proc Natl Acad Sci U S A, 104, 2007, p10865-10870<\/li>\n\n\n\n<li>Nanosculpting reversed wavelength sensitivity into a photoswitchable iGluR.<br>Numano R, Szobota S, Lau AY, Gorostiza P, Volgraf M, Roux B, Trauner D, Isacoff EY.<br>Proc Natl Acad Sci U S A, 106, 2009, p6814-6819<\/li>\n<\/ol>\n\n\n\n<h4 class=\"wp-block-heading\">\u30ad\u30fc\u30ef\u30fc\u30c9<\/h4>\n\n\n\n<p>LiGluR\u53d7\u5bb9\u4f53\u3001Yin\/Yang\u53d7\u5bb9\u4f53\u3001\u30a2\u30be\u30d9\u30f3\u30bc\u30f3\u69cb\u9020\u3001MAG\u3001iGluR6\u3001UV\u5149\u3067\u3001\u53ef\u8996\u5149<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\u907a\u4f1d\u5b50\u5c0e\u5165\u88c5\u7f6e<\/h3>\n\n\n\n<p>\u4e00\u822c\u306b\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6cd5\u3067\u907a\u4f1d\u5b50\u5c0e\u5165\u3092\u884c\u3046\u5834\u5408\u3001\u30ad\u30e5\u30d9\u30c3\u30c8\u3068\u547c\u3070\u308c\u308b\u3001\u4e8c\u3064\u306e\u5bfe\u306b\u306a\u308b\u96fb\u6975\u3092\u5099\u3048\u305f\u5bb9\u5668\u3092\u7528\u3044\u307e\u3059\u3002\u3053\u306e\u5bb9\u5668\u3078\u7d30\u80de\u3068\u5c0e\u5165\u3057\u305f\u3044\u907a\u4f1d\u5b50\u304c\u6df7\u5408\u3055\u308c\u305f\u6db2\u4f53\u3092\u52a0\u3048\u3001\u8a2d\u8a08\u3055\u308c\u305f\u96fb\u5727\u3092\u5370\u52a0\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u907a\u4f1d\u5b50\u5c0e\u5165\u3092\u884c\u3044\u307e\u3059\u3002\u3053\u306e\u624b\u6cd5\u3067\u306f\u3001\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u3092\u7528\u3044\u306a\u3044\u305f\u3081\u3001\u307b\u3068\u3093\u3069\u306e\u7814\u7a76\u5ba4\u3067\u7c21\u5358\u306b\u5c0e\u5165\u3067\u304d\u3001\u79fb\u690d\u306b\u8010\u3048\u3046\u308b\u30b0\u30ec\u30fc\u30c9\u3092\u9054\u6210\u3067\u304d\u307e\u3059\u3002\u307e\u305f\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u8a66\u85ac\u3092\u7528\u3044\u306a\u3044\u305f\u3081\u3001\u30e9\u30f3\u30cb\u30f3\u30b0\u30b3\u30b9\u30c8\u3092\u4f4e\u304f\u6291\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6cd5\u306b\u306f\u7d30\u80de\u6bd2\u6027\u304c\u6975\u3081\u3066\u9ad8\u3044\u3068\u8a00\u3063\u305f\u81f4\u547d\u7684\u306a\u5f31\u70b9\u304c\u3042\u308a\u307e\u3057\u305f\u3002\u73fe\u5728\u3001\u4f4e\u7d30\u80de\u6bd2\u6027\u3092\u5b9f\u73fe\u3059\u308b\u7269\u7406\u7684\u306a\u907a\u4f1d\u5b50\u5c0e\u5165\u6cd5\u306e\u958b\u767a\u304c\u6025\u304c\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u4e2d\u3067\u3082\u3001\u79c1\u305f\u3061\u304c\u7740\u76ee\u3057\u305f\u306e\u306f\u9759\u96fb\u6c17\u3067\u3057\u305f\u3002\u96fb\u5727\u3084\u96fb\u6d41\u304c\u9ad8\u3044\u306e\u306b\u96fb\u529b\u91cf\u304c\u4f4e\u3044\u9759\u96fb\u6c17\u306f\u9069\u5fdc\u7d30\u80de\u7a2e\u306e\u5e83\u3044\u907a\u4f1d\u5b50\u5c0e\u5165\u6cd5\u3092\u78ba\u7acb\u3067\u304d\u308b\u53ef\u80fd\u6027\u3092\u6301\u3063\u3066\u3044\u307e\u3059\u3002\u3067\u306f\u3069\u306e\u3088\u3046\u306b\u9759\u96fb\u6c17\u3092\u907a\u4f1d\u5b50\u5c0e\u5165\u6642\u306b\u4e0e\u3048\u308b\u304b\u306b\u3064\u3044\u3066\u307f\u3066\u3044\u304d\u307e\u3059\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"724\" src=\"https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/wo2-1024x724.jpg\" alt=\"\" class=\"wp-image-97\" srcset=\"https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/wo2-1024x724.jpg 1024w, https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/wo2-300x212.jpg 300w, https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/wo2-768x543.jpg 768w, https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/wo2.jpg 1086w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n\n\n<p>\u79c1\u305f\u3061\u304c\u9032\u3081\u308b\u7814\u7a76\u306f\u3001\u6cb9\u3068\u6c34\u304c\u5225\u308c\u308b\u3053\u3068\u3092\u5229\u7528\u3057\u3066\u3044\u307e\u3059\u3002\u307e\u305a\u3001\u7d76\u7e01\u6027\u306e\u6cb9\u3092\u6e80\u305f\u3057\u305f\u30b7\u30e3\u30fc\u30ec\u306e\u4e2d\u306b\u7d30\u80de\u3068\u6838\u9178\u3092\u6df7\u305c\u305f\u6eb6\u6db2\u3092\u843d\u3068\u3057\u307e\u3059\u3002\u6cb9\u306e\u4e2d\u3067\u843d\u3068\u3057\u305f\u6db2\u4f53\u304c\u7403\u72b6\u306b\u306a\u308a\u6db2\u6ef4\u304c\u5f62\u6210\u3055\u308c\u307e\u3059\u3002\u6b21\u306b\u3001\u6db2\u6ef4\u306e\u3067\u304d\u305f\u7d76\u7e01\u6cb9\u306e\u4e2d\u3078\u91d1\u5c5e\u88fd\u306e\u96fb\u6975\u3092\u633f\u5165\u3057\u307e\u3059\u3002\u3053\u306e\u96fb\u6975\u3078\u9759\u96fb\u6c17\u304f\u3089\u3044\u306e\u96fb\u5727\uff08\u6570kV\uff09\u3092\u5370\u52a0\u3059\u308b\u3068\u3001\u6db2\u6ef4\u306f\u521d\u671f\u5e2f\u96fb\u306e\u6975\u6027\u306b\u5f93\u3063\u3066\u52d5\u304d\u59cb\u3081\u307e\u3059\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"724\" src=\"https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/wo3-1024x724.jpg\" alt=\"\" class=\"wp-image-98\" srcset=\"https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/wo3-1024x724.jpg 1024w, https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/wo3-300x212.jpg 300w, https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/wo3-768x543.jpg 768w, https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/wo3.jpg 1086w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>\u6db2\u6ef4\u304c\u96fb\u6975\u307e\u3067\u5230\u9054\u3059\u308b\u3068\u3001\u96fb\u6975\u304b\u3089\u6db2\u6ef4\u306e\u5145\u96fb\u304c\u884c\u308f\u308c\u307e\u3059\u3002\u3053\u306e\u6642\u3001\u56f3\u306e\u3088\u3046\u306b\u3001\u521d\u671f\u5e2f\u96fb\u304c\u30de\u30a4\u30ca\u30b9\u306a\u3089\u3001\u6db2\u6ef4\u8868\u9762\u306b\u84c4\u3048\u3089\u308c\u305f\u96fb\u5b50\u304c\u96fb\u6975\u304b\u3089\u5438\u3044\u53d6\u3089\u308c\u3001\u30d7\u30e9\u30b9\u306b\u5e2f\u96fb\u3057\u307e\u3059\u3002\u3061\u3087\u3046\u3069\u3001\u30c9\u30a2\u30ce\u30d6\u306b\u624b\u3092\u89e6\u308c\u308b\u77ac\u9593\u30d1\u30c3\u30c1\u3063\u3068\u304f\u308b\u3088\u3046\u306a\u30a4\u30e1\u30fc\u30b8\u3067\u3059\u3002\u30d7\u30e9\u30b9\u306e\u5e2f\u96fb\u306b\u3088\u308a\u30de\u30a4\u30ca\u30b9\u96fb\u6975\u304b\u3089\u30af\u30fc\u30ed\u30f3\u529b\u306b\u3088\u308b\u5f15\u529b\u304c\u50cd\u304d\u3001\u6db2\u6ef4\u306f\u30de\u30a4\u30ca\u30b9\u96fb\u6975\u3078\u5411\u304b\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"724\" src=\"https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/wo4-1024x724.jpg\" alt=\"\" class=\"wp-image-99\" srcset=\"https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/wo4-1024x724.jpg 1024w, https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/wo4-300x212.jpg 300w, https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/wo4-768x543.jpg 768w, https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/wo4.jpg 1086w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>\u30de\u30a4\u30ca\u30b9\u96fb\u6975\u3078\u6db2\u6ef4\u304c\u5230\u9054\u3059\u308b\u3068\u3001\u96fb\u6975\u8868\u9762\u306e\u96fb\u5b50\u304c\u6db2\u6ef4\u8868\u9762\u3078\u306a\u3060\u308c\u8fbc\u307f\u3001\u30d7\u30e9\u30b9\u6975\u306b\u6db2\u6ef4\u304c\u63a5\u89e6\u3057\u305f\u6642\u3068\u540c\u69d8\u3001\u6db2\u6ef4\u306e\u5e2f\u96fb\u304c\u77ac\u6642\u306b\u5909\u5316\u3057\u307e\u3059\u3002\u4ee5\u4e0a\u306e\u3088\u3046\u306a\u7406\u8ad6\u3092\u7e70\u308a\u8fd4\u3057\u6db2\u6ef4\u304c\u5f80\u5fa9\u904b\u52d5\u3057\u307e\u3059\u3002\u6db2\u6ef4\u304c\u5f80\u5fa9\u904b\u52d5\u3059\u308b\u6700\u4e2d\u3067\u3001\u91d1\u5c5e\u96fb\u6975\u3078\u63a5\u89e6\u3059\u308b\u305f\u3073\u306b\u6db2\u6ef4\u306e\u5e2f\u96fb\u304c\u5909\u5316\u3057\u3001\u6db2\u6ef4\u4e21\u7aef\u9593\u306b\u9ad8\u3044\u96fb\u754c\u304c\u767a\u751f\u3057\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306b\u3057\u3066\u9759\u96fb\u6c17\u3092\u907a\u4f1d\u5b50\u5c0e\u5165\u306b\u5229\u7528\u3057\u3066\u304a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u5b9f\u969b\u306b\u3053\u306e\u88c5\u7f6e\u3092\u7528\u3044\u3066\u907a\u4f1d\u5b50\u5c0e\u5165\u3092\u884c\u3046\u3068\u3001\u591a\u7a2e\u306e\u7d30\u80de\u3078\u907a\u4f1d\u5b50\u5c0e\u5165\u3067\u304d\u308b\u3053\u3068\u304b\u3089\u3001\u3053\u306e\u77ed\u6642\u9593\u306e\u9ad8\u96fb\u754c\u306b\u3088\u308a\u907a\u4f1d\u5b50\u5c0e\u5165\u304c\u884c\u308f\u308c\u3066\u3044\u308b\u3068\u8003\u3048\u3066\u3044\u307e\u3059\u3002\u6db2\u6ef4\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6cd5\u306b\u3088\u308b\u907a\u4f1d\u5b50\u5c0e\u5165\u306f\u975e\u5e38\u306b\u7d30\u80de\u6bd2\u6027\u304c\u4f4e\u3044\u304c\u3001\u7d30\u80de\u6bd2\u6027\u306b\u5bfe\u3059\u308b\u907a\u4f1d\u5b50\u5c0e\u5165\u52b9\u7387\u304c\u9ad8\u3044\u7279\u5fb4\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"724\" src=\"https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/wo1-1024x724.jpg\" alt=\"\" class=\"wp-image-100\" srcset=\"https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/wo1-1024x724.jpg 1024w, https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/wo1-300x212.jpg 300w, https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/wo1-768x543.jpg 768w, https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/wo1.jpg 1086w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>\u5f53\u7814\u7a76\u5ba4\u306e\u898b\u89e3\u3067\u306f\u3001\u3053\u306e\u907a\u4f1d\u5b50\u5c0e\u5165\u52b9\u7387\u306e\u9ad8\u3055\u306f\u3001\u6db2\u6ef4\u3068\u3044\u3046\u5c0f\u3055\u306a\u53cd\u5fdc\u5834\u306b\u3088\u308a\u907a\u4f1d\u5b50\u5c0e\u5165\u304c\u884c\u3048\u308b\u3053\u3068\u304c\u3001\u95a2\u4fc2\u3057\u3066\u3044\u308b\u306e\u3067\u306f\u306a\u3044\u304b\u3068\u8003\u3048\u3066\u304a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u73fe\u5728\u3001\u6db2\u6ef4\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6cd5\u306e\u907a\u4f1d\u5b50\u5c0e\u5165\u52b9\u7387\u3092\u5411\u4e0a\u3055\u305b\u308b\u3053\u3068\u3084\u9069\u5fdc\u7d30\u80de\u7a2e\u3092\u5e83\u3052\u308b\u3053\u3068\u3092\u76ee\u7684\u306b\u3057\u305f\u907a\u4f1d\u5b50\u5c0e\u5165\u7cbe\u5ea6\u5411\u4e0a\u306e\u7814\u7a76\u3001\u6db2\u6ef4\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u3092\u518d\u751f\u533b\u7642\u306b\u5fdc\u7528\u3059\u308b\u305f\u3081\u306e\u7814\u7a76\u3001\u6db2\u6ef4\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u304b\u3089\u30d2\u30f3\u30c8\u3092\u5f97\u305f\u65b0\u3057\u3044\u65b9\u5f0f\u306e\u4f4e\u7d30\u80de\u6bd2\u6027\u907a\u4f1d\u5b50\u5c0e\u5165\u88c5\u7f6e\u306e\u958b\u767a\u3092\u884c\u306a\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\u7d30\u80de\u3078\u306e\u907a\u4f1d\u5b50\u5c0e\u5165\u306e\u9769\u65b0\u7684\u65b0\u6cd5\u300c\u6db2\u6ef4\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u300d\u306e\u958b\u767a<\/h3>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"987\" height=\"513\" src=\"https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/Sum4.jpg\" alt=\"\" class=\"wp-image-101\" srcset=\"https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/Sum4.jpg 987w, https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/Sum4-300x156.jpg 300w, https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-content\/uploads\/2023\/01\/Sum4-768x399.jpg 768w\" sizes=\"auto, (max-width: 987px) 100vw, 987px\" \/><figcaption class=\"wp-element-caption\">\u672c\u88c5\u7f6e\u306b\u3088\u308b\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u30b3\u30fc\u30c9\u907a\u4f1d\u5b50\u306e\u8907\u6570\u7a2e\u540c\u6642\u5c0e\u5165\uff08HEK293\uff09<\/figcaption><\/figure>\n\n\n\n<h4 class=\"wp-block-heading\">\u6db2\u6ef4\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u306b\u3088\u308b\u54fa\u4e73\u985e\u7d30\u80de\u3078\u306e\u5f62\u8cea\u5c0e\u5165<\/h4>\n\n\n\n<p>\u5f53\u7814\u7a76\u5ba4\u3067\u306f\u3001\u6db2\u6ef4\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u3068\u3044\u3046\u3001\u9769\u65b0\u7684\u306a\u7d30\u80de\u3078\u306e\u907a\u4f1d\u5b50\u5c0e\u5165\u6cd5\u3092\u958b\u767a\u3057\u305f\u3002\u7d76\u7e01\u4f53\u306e\u6cb9\u4e2d\u306e\u7d30\u80de\u3068\u5916\u6765\u6027DNA\u3092\u5c01\u5165\u3057\u305f\u6570\u30de\u30a4\u30af\u30ed\u30ea\u30c3\u30c8\u30eb\u306e\u6db2\u6ef4\u306b\u76f4\u6d41\u96fb\u754c\u3092\u5370\u52a0\u3059\u308b\u3068\u3001\u6cb9\u4e2d\u306e\u96fb\u6975\u9593\u3092\u6db2\u6ef4\u304c\u5f80\u5fa9\u904b\u52d5\u3059\u308b\u3002\u96fb\u6975\u306e\u63a5\u89e6\u6642\u306e\u96fb\u8377\u306e\u53cd\u8ee2\u6642\u3084\u3001\u77ed\u7d61\u73fe\u8c61\u304c\u751f\u3058\u308b\u9593\u306b\u3001\u5fae\u91cf\u306a\u96fb\u6d41\u304c\u5370\u52a0\u3055\u308c\u3001DNA\u304c\u7d30\u80de\u819c\u5185\u306b\u5c0e\u5165\u3055\u308c\u3001\u7d30\u80de\u306e\u751f\u7406\u6a5f\u80fd\u304c\u5909\u5316\u3059\u308b\u3002\u3053\u306e\u6280\u8853\u306f\u3001\u4f7f\u7528\u3059\u308b\u7d30\u80de\u3084DNA\u91cf\u3092\u5927\u5e45\u306b\u524a\u6e1b\u53ef\u80fd\u3067\u3042\u308a\u3001\u7d30\u80de\u3078\u306e\u6bd2\u6027\u304c\u4f4e\u304f\u3001\u30cf\u30a4\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u306e\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u304c\u53ef\u80fd\u3067\u3042\u308b\u3001\u3053\u306e\u6280\u8853\u3092\u7528\u3044\u3066\u3001iPS\u7d30\u80de\u306e\u91cf\u7523\u5316\u3084\u30de\u30eb\u30c1\u907a\u4f1d\u5b50\u6539\u5909\u306a\u3069\u533b\u5b66\u306b\u3082\u9769\u65b0\u3092\u3082\u305f\u3089\u3059\u3053\u3068\u304c\u3067\u304d\u308b\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">\u4e3b\u306a\u696d\u7e3e<\/h4>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Novel Parallelized Electroporation by Electrostatic Manipulation of a Water-in-Oil Droplet as a Microreactor.<br>Kurita H, Takahashi S, Asada A, Matsuo M, Kishikawa K, Mizuno A, Numano R.PLoS One. <strong>2015<\/strong> Dec 9;10(12):e0144254.<br>DOI: <a rel=\"noreferrer noopener\" href=\"https:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0144254\" data-type=\"URL\" data-id=\"https:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0144254\" target=\"_blank\">10.1371\/journal.pone.0144254<\/a><\/li>\n\n\n\n<li>Fundamental study on agenetransfectionmethodologyfor mammalian cellsusingwater-in-oildropletdeformationinaDC electric field<br>Kurita H, Takao Y, Kishikawa K, Takashima K, Numano R, Mizuno A.Biochemistry andBiophysicsReports, <strong>2016<\/strong>,8,p81\u201388<br>DOI:<a href=\"https:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0144254\" target=\"_blank\" rel=\"noreferrer noopener\">10.1016\/j.bbrep.2016.08.001<\/a><\/li>\n<\/ol>\n\n\n\n<h4 class=\"wp-block-heading\">\u30ad\u30fc\u30ef\u30fc\u30c9<\/h4>\n\n\n\n<p>\u6db2\u6ef4\u3001\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u3001\u5f62\u8cea\u5c0e\u5165\u3001iPS\u7d30\u80de\u306e\u91cf\u7523\u5316<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7814\u7a76\u5185\u5bb9 \u54fa\u4e73\u985e\u6982\u65e5\u30ea\u30ba\u30e0\u306e\u5206\u5b50\u6642\u8a08\u306e\u30e2\u30cb\u30bf\u30fc\u5b9f\u9a13\u7cfb\u306e\u78ba\u7acb Per1\uff1aluc\u306e\u7d44\u307f\u63db\u3048\u52d5\u7269\u306eSCN\u306e\u767a\u5149\u30ea\u30ba\u30e0 \u5730\u7403\u4e0a\u306e\u751f\u7269\u306e\u884c\u52d5\u3084\u4ee3\u8b1d\u306a\u3069\u306b\u306f\u7d04\u4e00\u65e5\u3092\u5468\u671f\u3068\u3059 &#8230; <\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-15","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-json\/wp\/v2\/pages\/15","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-json\/wp\/v2\/comments?post=15"}],"version-history":[{"count":7,"href":"https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-json\/wp\/v2\/pages\/15\/revisions"}],"predecessor-version":[{"id":164,"href":"https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-json\/wp\/v2\/pages\/15\/revisions\/164"}],"wp:attachment":[{"href":"https:\/\/www.eiiris.tut.ac.jp\/numano\/wp-json\/wp\/v2\/media?parent=15"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}