{"id":502,"date":"2025-10-06T14:02:27","date_gmt":"2025-10-06T05:02:27","guid":{"rendered":"https:\/\/www.eiiris.tut.ac.jp\/en\/?p=502"},"modified":"2025-11-26T13:29:16","modified_gmt":"2025-11-26T04:29:16","slug":"251001-paper","status":"publish","type":"post","link":"https:\/\/www.eiiris.tut.ac.jp\/en\/research-activities\/research-publication\/251001-paper\/","title":{"rendered":"3 million cells per minute: parallel microdevice with AI-powered single-cell analysis"},"content":{"rendered":"\r\n<p>\u3000Researchers at the Indian Institute of Technology (IIT) Madras and Toyohashi University of Technology (TUT) have developed an integrated platform that combines a massively parallel through-hole cell-squeezing mechanoporation device for high-throughput intracellular delivery, and an automated single-cell image-cytometry pipeline built on Mask R-CNN. The device guides cells through an array of up to 62,000 tiny through-holes that are narrower than the cell diameter. A brief, gentle squeeze creates transient membrane pores that admit biomolecules into the cell interior, then reseal, allowing cells to recover. The automated analysis system uses deep-learning to report cell size, the fraction of cargo-positive cells, the fraction of viable cells, and per-cell fluorescence intensity in about 83 seconds. By coupling scalable intracellular delivery with rapid, automated evaluation, the platform expands what can be accomplished in a single day\u2014from large-scale screening and diagnostics to practical cell manipulation and future point-of-care gene-editing workflows.<\/p>\r\n<p>&nbsp;<\/p>\r\n<p>Bibliographic Information<br \/>\u201cHigh Throughput Intracellular Delivery Using a 2D Cell-Squeezing Mechanoporation Device and Its Analysis by a Deep Learning Model\u201d,Pulasta Chakrabarty, Abinaya R, Ryoma Suzuki, Srikanth Vedantam, Suresh Rao, Moeto Nagai,<br \/>Tuhin Subhra SantraAdvanced Healthcare Materialse02472First published: 21 August 2025<br \/><br \/><\/p>\r\n<p>DOI:<a href=\"https:\/\/doi.org\/10.1002\/adhm.202502472\" target=\"_blank\" rel=\"noopener noreferrer\">https:\/\/doi.org\/10.1002\/adhm.202502472<\/a><\/p>\r\n<p>&nbsp;<\/p>\r\n<p>&nbsp;<\/p>\r\n<p><a href=\"https:\/\/www.eiiris.tut.ac.jp\/?attachment_id=7437\" rel=\"attachment wp-att-7437\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.eiiris.tut.ac.jp\/wp-content\/uploads\/2025\/10\/251001image1.png\" alt=\"\" width=\"595\" height=\"406\" class=\"alignnone size-full wp-image-7437\" \/><\/a>\u00a0<\/p>\r\n<p><a href=\"https:\/\/www.eiiris.tut.ac.jp\/?attachment_id=7438\" rel=\"attachment wp-att-7438\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.eiiris.tut.ac.jp\/wp-content\/uploads\/2025\/10\/251001image2.png\" alt=\"\" width=\"588\" height=\"511\" class=\"alignnone size-full wp-image-7438\" \/><\/a><br \/><br \/>\u00a0\u00a0\u00a0<\/p>\r\n<p>\uff1c\u95a2\u9023\u8a18\u4e8b\uff1e<br \/><a href=\"https:\/\/www.eurekalert.org\/news-releases\/1099713\" target=\"_blank\" rel=\"noopener noreferrer\">https:\/\/www.eurekalert.org\/news-releases\/1099713<\/a><\/p>\r\n<p>&nbsp;<\/p>\r\n<p>&nbsp;<\/p>\r\n","protected":false},"excerpt":{"rendered":"\u3000Researchers at the Indian Institute of Technology (IIT) Madras and Toyohashi University of Technology (TUT) h [&hellip;]","protected":false},"author":4,"featured_media":506,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"class_list":["post-502","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-research-publication"],"_links":{"self":[{"href":"https:\/\/www.eiiris.tut.ac.jp\/en\/wp-json\/wp\/v2\/posts\/502","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.eiiris.tut.ac.jp\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.eiiris.tut.ac.jp\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.eiiris.tut.ac.jp\/en\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/www.eiiris.tut.ac.jp\/en\/wp-json\/wp\/v2\/comments?post=502"}],"version-history":[{"count":5,"href":"https:\/\/www.eiiris.tut.ac.jp\/en\/wp-json\/wp\/v2\/posts\/502\/revisions"}],"predecessor-version":[{"id":516,"href":"https:\/\/www.eiiris.tut.ac.jp\/en\/wp-json\/wp\/v2\/posts\/502\/revisions\/516"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.eiiris.tut.ac.jp\/en\/wp-json\/wp\/v2\/media\/506"}],"wp:attachment":[{"href":"https:\/\/www.eiiris.tut.ac.jp\/en\/wp-json\/wp\/v2\/media?parent=502"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.eiiris.tut.ac.jp\/en\/wp-json\/wp\/v2\/categories?post=502"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}